GABA A Receptor alpha 1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
gamma-aminobutyric acid(GABA) A receptor, alpha 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1(440-456aa ATYLNREPQLKAPTPHQ), identical to the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	GABRA1
Protein	Gamma-aminobutyric acid receptor subunit alpha-1
Uniprot ID	P14867
Function	Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand- gated chloride channel (By similarity).
Tissue Specificity
Sub-cellular localization	Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence Similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.
Aliases	ECA4 antibody|EJM antibody|EJM5 antibody|Gaba receptor alpha 1 polypeptide antibody|GABA(A) receptor antibody|GABA(A) receptor subunit alpha 1 antibody|GABA(A) receptor subunit alpha-1 antibody|GABA(A) receptor, alpha 1 antibody|GABRA 1 antibody|GABRA1 antibody|Gamma aminobutyric acid(GABA) A receptor alpha 1 antibody|Gamma aminobutyric acid A receptor alpha 1 antibody|Gamma aminobutyric acid receptor subunit alpha 1 antibody|Gamma-aminobutyric acid receptor subunit alpha-1 antibody|GBRA1_HUMAN antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information