Fas Ligand Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
Fas ligand(TNF superfamily, member 6)	Polyclonal	IgG	Rabbit	Human	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(192-218aa YSKVYFRGQSCNNLPLSHKVYMRNSKY), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	FASLG
Protein	Tumor necrosis factor ligand superfamily member 6
Uniprot ID	P48023
Function	Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.
Tissue Specificity
Sub-cellular localization	Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Note: Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane.
Sequence Similarities	Belongs to the tumor necrosis factor family.
Aliases	ALPS1B antibody|APO1L antibody|Apoptosis(APO 1) antigen ligand 1 antibody|Apoptosis antigen ligand 1 antibody|Apoptosis antigen ligand antibody|APT1LG1 antibody|APTL antibody|CD178 antibody|CD178 antigen antibody|CD95 ligand antibody|CD95-L antibody|CD95L antibody|CD95L protein antibody|FAS antigen ligand antibody|Fas L antibody|Fas ligand(TNF superfamily member 6) antibody|Fas ligand antibody|FASL antibody|Fasl Fas ligand(TNF superfamily member 6) antibody|FASLG antibody|Generalized lymphoproliferative disease antibody|Gld antibody|soluble form antibody|TNF superfamily member 6 antibody|TNFL6_HUMAN antibody|TNFSF6 antibody|Tumor necrosis factor(ligand) superfamily member 6 antibody|Tumor necrosis factor ligand superfamily member 6 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information