Connexin 37/GJA4 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
gap junction protein, alpha 4, 37kDa	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 37(3-17aa DWGFLEKLLDQVQEH), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	GJA4
Protein	Gap junction alpha-4 protein
Uniprot ID	P35212
Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Tissue Specificity	Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
Sub-cellular localization	Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
Sequence Similarities
Aliases	Connexin 37 antibody|Connexin-37 antibody|Cx37 antibody|CXA4_HUMAN antibody|Gap junction alpha 4 protein antibody|Gap junction alpha-4 protein antibody|gap junction protein, alpha 4 37kDa(connexin 37) antibody|gap junction protein, alpha 4, 37kD(connexin 37) antibody|Gap junction protein, alpha 4, 37kDa antibody|Gja4 antibody|OTTHUMP00000004181 antibody|OTTHUMP00000004182 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolutio's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information