Cdc34 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
cell division cycle 34 homolog(S. cerevisiae)	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cdc34(148-163aa YRKWKESKGKDREYTD), identical to the related mouse and rat sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CDC34
Protein	Ubiquitin-conjugating enzyme E2 R1
Uniprot ID	P49427
Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5- mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.
Tissue Specificity	Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.
Sub-cellular localization	Cytoplasm. Nucleus. Note: The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.
Sequence Similarities	Belongs to the ubiquitin-conjugating enzyme family.
Aliases	Cdc 34 antibody|Cdc34 antibody|Cell division cycle 34 antibody|Cell division cycle 34 homolog(S. cerevisiae) antibody|Cell division cycle 34 homolog antibody|E2 CDC34 antibody|UB2R1_HUMAN antibody|UBC 3 antibody|UBC3 antibody|UBCH3 antibody|UBE2 R1 antibody|UBE2R1 antibody|Ubiquitin carrier protein antibody|Ubiquitin conjugating enzyme Cdc34 antibody|Ubiquitin conjugating enzyme E2 32 kDa complementing antibody|Ubiquitin protein ligase antibody|Ubiquitin protein ligase R1 antibody|Ubiquitin-conjugating enzyme E2 R1 antibody|Ubiquitin-conjugating enzyme E2-32 kDa complementing antibody|Ubiquitin-conjugating enzyme E2-CDC34 antibody|Ubiquitin-protein ligase R1 antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information