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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
Fc fragment of IgE, low affinity II, receptor for (CD23) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
E.coli-derived mouse CD23 recombinant protein (Position: E50-P331). Mouse CD23 shares 52% amino acid (aa) sequence identity with human CD23. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | FCER2 |
Protein | Low affinity immunoglobulin epsilon Fc receptor |
Uniprot ID | P20693 |
Function | Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). |
Tissue Specificity | |
Sub-cellular localization | Cell membrane ; Single-pass type II membrane protein . Cell membrane ; Lipid-anchor . |
Sequence Similarities | Contains 1 C-type lectin domain. |
Aliases | Blast 2 antibody|BLAST-2 antibody|Blast2 antibody|C type lectin domain family 4 member J antibody|C-type lectin domain family 4 member J antibody|C-type lectin domain family 4, member J antibody|CD 23 antibody|CD 23A antibody|CD23 antibody|CD23 antigen antibody|CD23A antibody|CLEC 4J antibody|CLEC4J antibody|Fc epsilon RII antibody|Fc fragment of IgE low affinity II receptor for antibody|Fc fragment of IgE, low affinity II, receptor for (CD23) antibody|Fc of IgE, low affinity II, receptor for (CD23) antibody|Fc receptor IgE low affinity II alpha polypeptide antibody|Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a antibody|Fc-epsilon-RII antibody|FCE 2 antibody|FCE2 antibody|FCER 2 antibody|Fcer2 antibody|FCER2_HUMAN antibody|FCER2A antibody|FceRII antibody|IgE binding factor antibody|IgE receptor lymphocyte antibody|IgE-binding factor antibody|IGEBF antibody|Immunoglobulin E binding factor antibody|Immunoglobulin E receptor, low affinity II antibody|Immunoglobulin E-binding factor antibody|LEUKOCYTE ANTIGEN CD23 antibody|Low Affinity IgE Receptor antibody|Low affinity immunoglobulin epsilon Fc receptor antibody|Low affinity immunoglobulin epsilon Fc receptor membrane bound form antibody|Low affinity immunoglobulin epsilon Fc receptor soluble form antibody|Ly-42 antibody|Ly42 antibody|Lymphocyte antigen CD23 antibody|Lymphocyte IgE receptor antibody|MGC93219 antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD23 antibody, ASA-B0366--1.jpg
IHC(P): Human Tonsil Tissue
Anti-CD23 antibody, ASA-B0366--2.jpg
IHC(P): Mouse Spleen Tissue
Anti-CD23 antibody, ASA-B0366--3.jpg
IHC(P): Rat Spleen Tissue