More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
CD1d molecule | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
A synthetic peptide corresponding to a sequence at the N-terminus of human CD1d(76-92aa FSDQQWETLQHIFRVYR). |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CD1D |
Protein | Antigen-presenting glycoprotein CD1d |
Uniprot ID | P15813 |
Function | Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. |
Tissue Specificity | Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. |
Sub-cellular localization | Cell membrane; Single-pass type I membrane protein. Endosome membrane. Lysosome membrane. Note: Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes. |
Sequence Similarities | |
Aliases | Antigen-presenting glycoprotein CD1d antibody|CD1.1 antibody|CD1A antibody|CD1d antibody|CD1D antigen antibody|CD1D antigen d polypeptide antibody|CD1d molecule antibody|CD1D_HUMAN antibody|Cd1d1 antibody|Differentiation antigen CD1 alpha 3 antibody|HMC class I antigen like glycoprotein CD1D antibody|Ly 38 antibody|MGC34622 antibody|R3 antibody|R3G1 antibody|T cell surface glycoprotein CD1d antibody|T cell surface glycoprotein CD1d precursor antibody|Thymocyte antigen CD1D antibody |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD1d antibody, ASA-B0362, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: HT1080 Cell Lysate
Lane 4: JURKAT Cell Lysate
Anti-CD1d antibody, ASA-B0362, IHC(P)
IHC(P): Human Mammary Cancer Tissue