More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystathionine-beta-synthase | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human CBS recombinant protein (Position: A331-K551). Human CBS shares 83% amino acid (aa) sequence identity with both mouse and rat CBS. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CBS |
Protein | Cystathionine beta-synthase |
Uniprot ID | P35520 |
Function | Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury (By similarity). |
Tissue Specificity | In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | Belongs to the cysteine synthase/cystathionine beta- synthase family. |
Aliases | AI047524 antibody|AI303044 antibody|Beta thionase antibody|Beta-thionase antibody|Cbs antibody|Cbs cystathionine beta-synthase antibody|CBS_HUMAN antibody|Cystathionine beta synthase antibody|Cystathionine beta-synthase antibody|EC 4.2.1.22 antibody|HIP 4 antibody|HIP4 antibody|Methylcysteine synthase antibody|MGC18856 antibody|MGC18895 antibody|MGC37300 antibody|OTTHUMP00000109416 antibody|OTTHUMP00000109418 antibody|Serine sulfhydrase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CBS antibody, ASA-B0325, Western blotting
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
WB: Recombinant human CBS Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
WB: Recombinant human CBS Protein 0.5ng
Predicted bind size: 42KD
Observed bind size: 42KD
Anti- CBS antibody, ASA-B0325, Western blotting
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Hela Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti CBS (ASA-B0325) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Hela Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: Hepg2 Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Anti- CBS antibody, ASA-B0325, IHC(P)
IHC(P): Human Liver Cancer Tissue
IHC(P): Human Liver Cancer Tissue