Calretinin Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
calbindin 2	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human Calretinin(234-262aa EMNIQQLTNYRKSVMSLAEAGKLYRKDLE), different from the related mouse and rat sequences by one amino acid.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CALB2
Protein	Calretinin
Uniprot ID	P22676
Function	Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Tissue Specificity	Brain.
Sub-cellular localization
Sequence Similarities	Belongs to the calbindin family.
Aliases	29 kDa calbindin antibody|CAB 29 antibody|CAB29 antibody|CAL 2 antibody|CAL2 antibody|CALB 2 antibody|CALB2 antibody|CALB2_HUMAN antibody|Calbindin 2 29kDa antibody|Calbindin 2 antibody|Calbindin D29K antibody|Calbindin2 antibody|Calretinin antibody|CR antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse, Rat Human	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Mouse, Rat Human	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information