More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
calbindin 1, 28kDa | Monoclonal | IgG | Mouse | Bovine, Human, Mouse, Rat | IHC-P, WB | Ascites |
Immunogen |
Bovine kidney calbindin-D |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CALB1 |
Protein | Calbindin |
Uniprot ID | Human(P05937), Mouse(P12658), Rat(P07171) |
Function | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the calbindin family. |
Aliases | CALB|D-28K|CALBINDIN, 28-KD|Calbindin D28|Calbindin D|Vitamin D-dependent calcium-binding protein, avian-type |
Application Details
Application | Concentration* | Species | Validated Using** |
Western blot | 0.5-1μg/ml | Human, bovine, mouse, rat | AssaySolutio's ECL kit |
Immunohistochemistry(Paraffin-embedded Section) | 1-2μg/ml | Human, bovine, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Calbindin-D antibody (monoclonal), ASA-B0263, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Anti-Calbindin-D antibody (monoclonal), ASA-B0263, IHC(P)
IHC(P): Mouse Kidney Tissue
Anti-Calbindin-D antibody (monoclonal), ASA-B0263, IHC(P)
IHC(P): Rat Kidney Tissue