beta-Actin Antibody (monoclonal)

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
actin, beta	Monoclonal	IgG	Mouse	Human, Mouse, Rat	IHC-P, WB	Ascites
Immunogen
slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.
Concentration	1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ACTB
Protein	Actin, cytoplasmic 1
Uniprot ID	Human(P60709), Mouse(P60710), Rat(P60711)
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Tissue Specificity
Sub-cellular localization	Cytoplasm, cytoskeleton.
Sequence Similarities	Belongs to the actin family.
Aliases	BRWS1|PS1TP5BP1|BETA-ACTIN|ACTIN, CYTOPLASMIC, 1

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.25-0.5μg/ml	Human, mouse, rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, mouse, rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information