More info
Assay Range | 125-8000 pg/mL |
Sensitivity | 10.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | cell lysates |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human HIF-1 alpha standard: 2 vials
2. One 96-well plate coated with Human HIF-1 alpha Ab
3. Sample diluent buffer: 25mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic Solution A: 6 mlx1
9. Chromogenic Solution B: 6 mL x1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
Hypoxia-inducible factor 1-alpha, also known as HIF-1 alpha, is a protein encoded by the HIF1A gene in humans. HIF1 is a heterodimeric basic helix-loop-helix transcription factor composed of Hif1a, the alpha subunit and the aryl hydrocarbon receptor nuclear translocator (Arnt), the beta subunit. HIF-1 alpha plays an essential role in cellular and systemic responses to hypoxia. Under hypoxic conditions, stabilized HIF1 alpha translocates to the nucleus and promotes the transcription of a host of genes that enable the cell to adapt to the lack of oxygen. HIF1 alpha promotes angiogenesis and the switch from aerobic respiration to anaerobic glycolysis. Many of the HIF1 alpha responsive genes encode proteins that promote glycolysis and/or inhibit oxidative phosphorylation.
PRODUCT CITATIONS
Effects of intermittent hypoxia and hyperoxia on angiogenesis and lung development in newborn mice
Elberson, V.D., Nielsen, L.C., Wang, H., Kumar, H.S.V
Journal of Neonatal-Perinatal Medicine, vol. 8, no. 4, pp. 313-322, 2016