More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 4.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human uPAR standard: 2 vials
2. One 96-well plate precoated with anti-Human uPAR Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
The Urokinase receptor, also known as uPA receptor, monocyte activation antigen Mo3, uPAR or CD87, is a highly glycosylated integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor and a receptor for urokinase-type plasminogen activator (uPA). The mature uPAR is a 283 amino acid (aa) protein derived from the full-length 335 aa uPAR precursor after removing the 22 aa signal peptide and a 30 aa fragment from the C-terminus during addition of the GPI anchor. uPAR, uPA and plasminogen activator inhibitor-1 (PAI-1) form a heterotrimetic complex that has exhibited diverse functions in regulation of cell attachment, migration, proliferation and differentiation, by both proteolytic and nonproteolytic mechanisms. There is a negative-feedback regulation by which uPAR mediates proteolysis-independent signal transduction of uPA and, in turn, uPA cleaves uPAR into an inactive form. uPAR has been involved in various non-proteolytic processes related to cancer, cell migration, cell cycle regulation, and cell adhesion.