More info
Assay Range | 156-10,000 pg/mL |
Sensitivity | 30 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample types | Cell/Tissue lysates |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Her3/ErbB3 standard: 2 vials
2. One 96-well plate coated Human Her3/ErbB3 Ab
3. Sample Diluent buffer : 12 mlx2
4. Antibody Diluent buffer : 12 mlx1
5. Streptinavidin-HRP Diluent Buffer: 12 mlx1
6.Biotinylated Human Her3/ErbB3 Ab: 1 vial
7. Streptinavidin-HRP: 1 vial
8.Chromogenic Solution A : 6 ml.
9. Chromogenic Solution B : 6 ml.
10. Stop solution: 6 mL x1
11.. Washing solution (20x): 25 mL x1
Background
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors.