More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Kallistatin standard: 2 vials.
2. One 96-well plate precoated with anti- Human Kallistatin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Kallistatin, also known as kallikrein inhibitor, peptidase inhibitor 4, PI-4, or serpin A4, is a member of the Serpin superfamily of the serine protease inhibitors. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. While most serpins control proteolytic cascades, certain serpins do not inhibit enzymes, but instead perform diverse functions such as storage (ovalbumin, in egg white), hormone carriage proteins (thyroxine-binding globulin, cortisol-binding globulin) and molecular chaperones (HSP47). Kallistatin is expressed by the liver and secreted in plasma. Kallistatin functions as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth. It can form a complex with tissue kallikrein to inhibit tissue kallikrein’s activity. Heparin blocks kallistatin's complex formation with tissue kallikrein and abolishes its inhibitory effect on tissue kallikrein's activity.