More info
Assay Range | 15.6 -10,00 pg/mL |
Sensitivity | 1.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human phospho- Lyn (Y397) standard: 2 vials
2. One 96-well plate coated Human phospho- Lyn (Y397) Ab
3. Diluent buffer 1: 12 ml
4. Diluent buffer 2: 12 ml
5. Diluent Buffer 3: 12 ml
6. Diluent Buffer 4: 12 ml
7.Biotinylated Human Phospho- Lyn (Y397) Ab: 1 vial
8. Streptinavidin-HRP: 1 vial
9.Chromogenic Solution A : 6 ml.
10. Chromogenic Solution B : 6 ml.
11. Stop solution: 6 mL x1
12. Washing solution (20x): 25 mL x1
Background
Lyn (Lck/Yes-related novel protein tyrosine kinase), also known as Tyrosine-protein kinase Lyn, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn,is a member of the Src family of protein tyrosine kinases. Lyn is mainly expressed in hematopoietic cells, neural tissues, liver, and adipose tissue. Functioned as a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors, Lyn plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, as well as responses to DNA damage and genotoxic agents. Lyn deficient mice display a phenotype that includes splenomegaly, a dramatic increase in numbers of myeloid progenitors and monocyte/macrophage tumors. Mice that expressed a hyperactive Lyn allele were tumor free and displayed no propensity toward hematological malignancy. These mice have reduced numbers of conventional B lymphocytes, down-regulated surface immunoglobulin M and costimulatory molecules, and elevated numbers of B1a B cells.