More info
Assay Range | 156 -10,000 pg/mL |
Sensitivity | 10 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Human phospho-GSK-3 alpha (S21) standard: 2 vials
2. One 96-well plate coated Human phospho-GSK-3 alpha (S21) Ab
3. Diluent buffer 1: 12 ml
4. Diluent buffer 2: 12 ml
5.Biotinylated Human Phospho-GSK-3 alpha (S21)Ab: 1 vial
6. Streptinavidin-HRP: 1 vial
7.Chromogenic Solution A : 6 ml.
8. Chromogenic Solution B : 6 ml.
9. Stop solution: 6 mL x1
10. Washing solution (20x): 25 mL x1
Background
Glycogen Synthase Kinase 3 (GSK-3) is a member of the Ser/Thr kinase family. GSK-3 functions as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally differently. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimers disease, bipolar disorder, and cancer. It is reported that homozygous disruption of the GSK-3β locus in mice results in embryonic lethality during mid-gestation