More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human P-Cadherin standard: 2 vials
2. One 96-well plate precoated with anti-Human P-Cadherin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
P Cadherin (Placental Cadherin, PCAD), also known as Cadherin-3, is a member of the Cadherin family. Binding to one another in a homophilic manner, Cadherins are calcium-dependent transmembrane proteins consisting of a large extracellular domain with DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single pass transmembrane domain, and a short carboxyl-terminal cytoplasmic domain responsible for interacting with the catenins. By interacting with the three catenins, α, β, and γ, Cadherins are linked to the cytoskeleton. This interaction is required for the cadherins’ adhesive activity. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. The full-length human P Cadherin is an 829 amino acid (aa) precursor protein with a 26 aa signal peptide and an 803 aa propeptide which can be further cleaved into the mature protein containing a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human mature PCAD is 87% aa identical to the mouse counterpart.