Human MMP-8 ELISA Kit

Kit Components

 1. Recombinant Human MMP-8 standard: 2 vials

 2. One 96-well plate precoated with anti- Human MMP-8 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Matrix metalloproteinase-8 (MMP-8), also known as gelatinase 2, is a member of the matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. Matrix Metalloproteinases (MMPs), also called Matrixins, plays an important role in the degradation of the extracellular matrix which is involved in a series of physiological processes related to embryonic development, morphogenesis, and tissue remodeling. MMPs have also been implicated in the pathogenesis of many diseases such as arthritis, cancer and cardiovascular disease. Human MMP-8 is secreted as a non-glycosylated proenzyme (pro-MMP-8) composed of a propeptide with a cysteine switch motif that is conserved in MMPs and keeps MMP-8 in an inactive state, and a mature polypeptide which consists of a catalytic domain with a zinc-binding motif conserved in metzincins. Activation of the mature MMP-8 is achieved by proteolytic removal of the propeptide or in vitro by proteases such as itself, MMP-3, MMP-10, and cathepsin G, and by chemicals such as mercury compounds and oxygen radicals. The active MMP-8 can degrade types I, II and III triple-helical collagen as well as itself. In addition, the active MMP-8 can cleave natural proteins such as fibronectin, cartilage aggrecan and serpins and peptides such as angiotensin and substance P.