More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 20.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human MIF standard: 2 vials.
2. One 96-well plate precoated with anti- Human MIF Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase, is a pro-inflammatory cytokine encoded by the MIF gene in humans. MIF is broadly expressed in various cell types and tissues including monocytes/macrophages, T and B cells, neutrophils, atherosclerotic foam cells, endothelium, smooth muscle, cardiomyocytes, tumor neovasculature and endocrine tissues. It is showed that MIF is involved in the innate immune response to bacterial pathogens. Bacterial antigens stimulate white blood cells to release MIF into the blood stream. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. In addition, glucocorticoids also stimulate white blood cells to release MIF which partially counteracts the inhibitory effects that glucocorticoids have on the immune system. On the other hand, MIF binds the chemokine receptors CXCR4 and/or CXCR and initiates a series of chemokine-like effects. MIF also exhibits receptor- independent uptake and signaling, binding intracellular Jab1 and relieving Jab1-mediated G1 cell cycle arrest. Clinically, elevated levels of MIF in the plasma of patients with sepsis or septic shock, in patients with acute respiratory distress syndrome, and in the synovial fluid of rheumatoid arthritis (RA) patients have been observed. MIF also shows intrinsic tautomerase and oxidoreductase activity in vitro, but the biological significance of the enzymatic activity is unknown.