More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human MICA standard: 2 vials
2. One 96-well plate precoated with anti-Human MICA Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
MICA (MHC class I chain-related gene A or MHC class I polypeptide-related sequence A) is a transmembrane glycoprotein encoded by the MICA gene in humans. Although MICA contains three extracellular Ig-like domains, it has no capacity to bind peptide or interact with β2-microglobulin. MICA is expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. MICA is a ligand for the KLRK1/NKG2D, an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells. MICA interacts with the cell surface receptor complex of KLRK1/NKG2D and HCST/DAP10. KLRK1 binds MICA directly, while HCST acts as an adapter molecule for signal transduction. In addition, MICA can interact with PDIA6 on the surface of tumor cells, leading to disulfide bond reduction which is required for release of MICA from tumor cells. MICA signaling has been associated with tumor surveillance, viral infections, and autoimmune diseases.