More info
Assay Range | 31.2-2,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma, body fluids, tissue lysate or cell culture supernatant |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human LYVE-1 standard: 2 vials
2. One 96-well plate coated with Human LYVE-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Lymphatic vessel endothelial hyaluronan (HA) receptor 1 (LYVE-1), also known as cell surface retention sequence-binding protein 1 (CRSBP-1), Extracellular link domain-containing protein 1, Hyaluronic acid receptor, is a ligand-specific transporter trafficking between intracellular organelles and the plasma membrane. LYVE-1also is a receptor of hyaluronan (HA), a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and Nacetyl D-glucosamine. HA exists in the extracellular matrix of many different tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. LYVE-1 shares 41% homology with CD44, another HA receptor. Human LYVE-1 shows 69% amino acid sequence identity with mouse LYVE-1. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes.