More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-29 standard: 2 vials
2. One 96-well plate precoated with anti- Human IL-29 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Interleukin-29 (IL-29), also known as Interferon lambda-1 (IFN-lambda-1), or cytokine Zcyto21, is a cytokine encoded by the IL29 gene in humans. Human IL-29 is synthesized as a 200 amino acid (aa) precursor protein containing a putative 19 aa signal peptide and a 181 aa mature protein. Structurally and functionally, IL-29 is closely related to IL-28A/ IFNλ2 and IL-28B/ IFNλ3. IL-29 shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively. The expression of these proteins is induced by virus infection or double stranded RNA. They have shown antiviral activity and upregulation of MHC class I antigen expression. These proteins elicit their effects through the same heterodimeric receptor complex that is composed of the IL-10 receptor β (IL-10 Rβ) and IL-28 Rα/IFN-λ R1. Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN regulatory factor 9 (IRF9) to form the IFN stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus and binds to the IFN stimulated response element (ISRE) present in the regulatory region of the target genes to regulate gene expression.