More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-27 standard: 2 vials.
2. One 96-well plate precoated with anti- Human IL-27 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits: Epstein-Barr virus (EBV)-induced gene 3 protein (EBI3) / IL-27B and p28 / IL-30. The human EBI3 is synthesized as a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region is composed of two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif. Human EBI3 exhibits 61% aa identical to mouse EBI3. The human p28 is a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region which is characterized by the presence of four αhelices featured also by the IL-6 family members. Human p28 shares 74% aa sequence identity with mouse counterpart.
IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to a heterodimeric receptor complex of WSX1/TCCR and gp130 to initiate a series of biological processes. IL-27 has exhibited multiple activities in immunoregulation. For instance, it can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, and plays a role in inducing isotype switching in B-cells. It also triggers interferon-gamma/IFN-gamma production in naive CD4 T-cells by cooperating with IL-12.