More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-24 standard: 2 vials
2. One 96-well plate precoated with anti-Human IL-24 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene 7) or suppression of tumorigenicity 16 protein, is a member of the IL-10 family. Human IL-24 is synthesized as a 206 amino acid (aa) precursor protein containing a signal sequence and an 18 kDa mature segment. There are three potential N-linked glycosylation sites in IL-24, and glycosylation is essential to its activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat counterparts and shows species cross-reactivity in rodent systems. IL-24 is expressed in a variety of cells including B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells.
IL-24 interacts with two heterodimeric receptors: IL-20Rα/IL-20Rβ and IL-22R1/IL-20Rβ to transduce its signals in target cells. IL-24 has exhibited multiple functions in inducing production of proinflammatory cytokines such as IFN-γ, IL-1β, IL-12 and TNFα, regulating cell survival and proliferation by activating the transcription factors STAT1 and STAT3 as well as anti-angiogenic activity.