More info
Assay Range | 15.6--1000 pg/mL |
Sensitivity | 3.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma or cell culture supernatant |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-15 standard: 2 vials
2. One 96-well plate coated with Human IL-15 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
IL -15 is a glycoprotein encoded by IL-15 gene in human. Human IL-15 is synthesized as a 162 amino acid (aa) precursor protein composed of a 48 aa signal peptide and a 114 aa mature fragment. In humans, an alternatively spliced form has also been identified. Human IL-15 shares approximately 73% aa sequence identity with mouse IL-15 and is active on mouse cells. The IL-15 receptor complex consists of three molecules: an IL-15 specific α-chain, a β-chain and γ-chain that is shared by the receptor complex for IL-2. The α-chain is a 237 aa protein and binds IL-15 with high affinity. It shows 54% aa sequence identity with mouse IL-15 Rα. Although the IL-15 receptor is composed of 3 chains, only the βγ dimer is required for IL-15 signaling. IL-15 plays various roles in immunoregulation. It stimulates the growth of natural killer cells, activated peripheral blood T lymphocytes, tumor infiltrating lymphocytes (TILs), and B cells. In addition, IL-15 also is a chemoattractant for human blood T lymphocytes and to induce both lymphokine-activated killer (LAK) activity in NK cells and the generation of cytolytic effector cells.