Data sheet
| Assay Range | 46.875 - 3000 pg/ml |
| Sensitivity | 46.8 pg/ml |
| Size | 96 T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative detection of Human ICAM-2. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Culture Supernates, Serum, plasma, body fluids, tissue lysates |
More info
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human ICAM-2 standard: 3 ng/vial - 2.
2. One 96-well plate precoated with anti-human ICAM-2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human ICAM2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Intercellular adhesion molecule 2 (ICAM2), also known as CD102, is a member of the intercellular adhesion molecule (ICAM) family. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response; NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.B4, also known as SLAMF4 or CD244, is a transmembrane protein belonging to the Ig superfamily.
