More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human FAP standard: 2 vials
2. One 96-well plate precoated with anti- Human FAP Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Fibroblast activation protein alpha (FAP), also known as seprase or 170 kDa melanoma membrane-bound gelatinase, is a type II transmembrane protein belonging to serine protease family. FAP is structurally and functionally related to dipeptidyl peptidase IV. FAP can degrade substrates with N-terminal Xaa Pro sequences. FAP also functions as an endopeptidase to degrade gelatin and Type I collagen. FAP is not detectable in normal tissues or resting fibroblasts, but is induced and highly expressed on reactive stromal fibroblasts in epithelial cancers, in granulation tissue during wound healing, and in bone and soft tissue sarcomas. Based on its expression patterns and enzymatic activities, it is proposed that FAP may participate in tumor invasion, tissue remodeling, and wound repair.