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Human Contactin 5 / CNTN5 ELISA Kit

NS-E10052

$599.00

Data sheet

Assay Range 62.5 - 4000 pg/ml

More info

Sensitivity
50 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 

Item Name

Size & Quantity

Storage

Contactin-5 standard (lyophilized)

4 ng/vial ×2

-70 °C or -20 °C

Detection antibody

1 vial, dilution 1:60, 180 µL

4 °C

Streptavidin-HRP

1 vial dilution 1:40, 300 µL

4 °C

96-well plate pre-coated with anti-Contactin-5 Ab

1

4 °C

Sample Diluent buffer

12 ml x 2

4 °C

Antibody diluent buffer

12 ml

4 °C

Streptavidin-HRP Diluent buffer

12 ml

4 °C

Chromogen Solution A (H2O2)

6 ml

4 °C

Chromogen Solution B (TMB)

6 ml

4 °C

Stop Solution

6 ml

4 °C

20 × Wash Buffer

25 ml

4 °C

Plate sealer

2

 

Manual

1

 

 

Background

 

Contactin-5 (CNTN5), also known as NB-2, is a membrane-anchored neuronal protein and also an adhesion molecule. CNTN5 is one of the neural recognition molecules in the contactin subgroup, which belongs to the immunoglobulin superfamily. Expression of NB-2 mRNA was restricted to cerebrum, cerebellum and was hardlty detectable, if any, in spinal cord. CNTN5 may play a role in the developing nervous system. NB-2 promoted neurite outgrowth of the cerebral cortical neurons but not the hippocampal neurons. 


Standard Curve

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