NS-E10052
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Assay Range | 62.5 - 4000 pg/ml |
Item Name |
Size & Quantity |
Storage |
Contactin-5 standard (lyophilized) |
4 ng/vial ×2 |
-70 °C or -20 °C |
Detection antibody |
1 vial, dilution 1:60, 180 µL |
4 °C |
Streptavidin-HRP |
1 vial dilution 1:40, 300 µL |
4 °C |
96-well plate pre-coated with anti-Contactin-5 Ab |
1 |
4 °C |
Sample Diluent buffer |
12 ml x 2 |
4 °C |
Antibody diluent buffer |
12 ml |
4 °C |
Streptavidin-HRP Diluent buffer |
12 ml |
4 °C |
Chromogen Solution A (H2O2) |
6 ml |
4 °C |
Chromogen Solution B (TMB) |
6 ml |
4 °C |
Stop Solution |
6 ml |
4 °C |
20 × Wash Buffer |
25 ml |
4 °C |
Plate sealer |
2 |
|
Manual |
1 |
|
Contactin-5 (CNTN5), also known as NB-2, is a membrane-anchored neuronal protein and also an adhesion molecule. CNTN5 is one of the neural recognition molecules in the contactin subgroup, which belongs to the immunoglobulin superfamily. Expression of NB-2 mRNA was restricted to cerebrum, cerebellum and was hardlty detectable, if any, in spinal cord. CNTN5 may play a role in the developing nervous system. NB-2 promoted neurite outgrowth of the cerebral cortical neurons but not the hippocampal neurons.