Data sheet
| Assay Range | 93.8 - 6000 pg/ml |
More info
Sensitivity
90 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CEACAM1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human CEACAM1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CEACAM1 Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) (CEACAM1) also known asCD66a (Cluster of Differentiation 66a), is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM1mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup.In melanocytic cells CEACAM1 gene expression may be regulated by MITF. In addition, CEACAM1 has been shown to interact with PTPN11 and Annexin A2.
