More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human CCL27 standard: 2 vials
2. One 96-well plate precoated with anti-Human CCL27 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Chemokine (C-C motif) ligand 27 (CCL27), also known as cutaneous T-cell-attracting chemokine (CTACK), ESkine, IL-11 R-alpha-locus chemokine, Skinkine, or small-inducible cytokine A27, is a small cytokine belonging to the CC chemokine family. Human CTACK is synthesized as a 112 amino acid (aa) precursor protein with a 24 aa signal sequence and a 88 aa mature protein. The mature human CCL27 is 84% aa identical to its mouse counterpart. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin.
CCL27 binds to the chemokine receptor CCR10 which also is a receptor for CCL28 and the broad-spectrum CC chemokine receptor antagonist vMIP-II from human herpes virus-8. CCL27 exhibits chemotactic activity for skin-associated memory T-lymphocytes and play a role in mediating homing of lymphocytes to cutaneous site. Increasing body of evidence indicated that normal skin-associated immuno-surveillance, as well as healthy and pathological cutaneous inflammatory responses are influenced by the CCL27/CCR10 interaction.