More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human CCL25 standard: 2 vials
2. One 96-well plate precoated with anti- Human CCL25 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
C-C motif chemokine 25 (CCL25), also known as small-inducible cytokine A25 or Thymus-expressed chemokine (TECK), is a small cytokine belonging to the CC chemokine family. CCL25 is expressed in thymus, small intestine, and small bowel. In the thymus, CCL25 is produced by the major CD11b- CD11c+ dendritic cell population. CCL25 is a ligand for the cytokine receptor CCR9. It is reported that CCR9 is expressed on thymocytes, gamma/delta T cells, immature dendritic cells, lamina propria mononuclear cells in the small bowel, gut homing CD4+ and CD8+ memory T cells that co-express the α4β7 integrin molecule, melanoma cells and melanoma cell lines and a variety of tumor cell lines. Except for CCR9, CCL25 also binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. CCL25 may play a role in T-cell development. Recombinant CCL25 exhibits chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. In addition to its role in cell migration, CCL25-induced signaling through CCR9 exhibits an anti-apoptosis regulatory function. For example, CCL25 induces resistance to TNF-α-mediated apoptosis in MOLT-4, T-ALL and T-CLL cells perhaps by induction of the inhibitor of apoptosis Livin. CCL25 may also participate in cycloheximide-mediated apoptosis. Clinically, increase in CCR9+ lymphocytes and CCL25 expression in the inflamed bowel has been associated with the pathogenesis of Crohn’s disease.