More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human CCL19 standard: 2 vials
2. One 96-well plate coated with anti- Human CCL19 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Macrophage inflammatory protein-3β (MIP3β), also known as chemokine (C-C motif) ligand 19 (CCL19), EBI1 ligand chemokine (ELC ), is a small cytokine belonging to the CC chemokine family. MIP3β is synthesized as a 98 amino acid (aa) residue precursor protein containing a predicted 21 aa residue signal peptide and a 77 aa residue mature protein. MIP3β is constitutively expressed in various lymphoid tissues including thymus, lymph nodes, appendix and spleen. MIP3β is a unique ligand for the chemokine receptor CKR7. MIP3β plays a role in normal lymphocyte recirculation and homing, trafficking of T cells in thymus, and in migration of T cell and B cell to secondary lymphoid organs.