Data sheet
| Assay Range | 18.75 - 1200 pg/ml |
| Sensitivity | 10 pg/ml |
| Size | 96T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of human ApolipoproteinH. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Serum, plasma, body fluids, tissue lysates. |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant Apolipoprotein H standard: 1.2ng/vial - 2.
2. One 96-well plate precoated with anti-Apolipoprotein H antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-Apolipoprotein H antibody: 1 vial, dilution 1:4000.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Apolipoprotein H (Apo-H), previously known as (β2-glycoprotein I, beta-2 glycoprotein I), is a multifunctional apolipoprotein. One of its functions is to bind cardiolipin. When bound the structure of cardiolipin and Apo-H both undergo large changes in structure. Apo-H also involves in phospholipid binding and agglutination. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk.
