More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human ACE standard: 2 vials
2. One 96-well plate precoated with anti- Human ACE Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Angiotensin I Converting Enzyme (ACE), also known as peptidyl-dipeptidase A or CD143, is a zinc metallopeptidase which is capable of releasing a C-terminal dipeptide featured with oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Therefore, it can cleave the C-terminal dipeptide from angiotensin I to produce the octapeptide angiotensin II, a potent vasopressor. It also inactivates the potent vasodilator, bradykinin, by the sequential removal of two C-terminal dipeptides. Two alternatively spliced ACE isoforms have been identified. The somatic ACE (sACE), found in endothelial, epithelial and neuronal cells, is made up of two highly similar active domains termed N- and C-domains containing the HExxH consensus sequence for zinc binding. The germinal ACE (gACE), found exclusively in the testes, is composed of a gACE-specific 67 amino acid sequence at N-terminus and a single catalytically active domain identical to the C-domain of sACE. Physiological functions of the two tissue-specific isozymes are not interchangeable. ACE plays an important role in blood pressure control and water and salt metabolism. In addition, it has showed diverse functions in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid β peptide (Aβ), retards Aβ aggregation, deposition, fibril formation, and inhibits cytotoxicity.