More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human CD244 standard: 2 vials
2. One 96-well plate precoated with anti- Human CD244 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
2B4, also known as, natural killer cell receptor 2B4, NK cell activation-inducing ligand (NAIL), NK cell type I receptor protein 2B4 (NKR2B4), CD244 and SLAMF4, is a type I transmembrane glycoprotein belonging to the SLAM subgroup of the CD2 protein family. SLAM family members consist of an extracellular domain (ECD) with two or four Ig like domains and at least two cytoplasmic receptor tyrosine based switch motifs (ITSMs). Mature human 2B4 consists of a 208 amino acid (aa) ECD with two Ig-like domains, a 21 aa transmembrane segment, and a 120 aa cytoplasmic domain with four ITSMs. The ECD of human 2B4 shares 46% and 40% aa sequence identity with mouse and rat 2B4, respectively. 2B4 is expressed on all NK cells, γδ T cells, monocytes, some CD4+ and CD8+ T cells, and dendritic cells. 2B4 is a high affinity ligand for CD48. 2B4/CD48 mediated signaling pathways are essential for regulation of natural killer cells and T lymphocyte functions. 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. And the inhibitory effect is associated with the long form of 2B4, while the activation is associated with the short form.