Human TNFsR ELISA Kit

Human TNFsR ELISA Kit

FM-E100139

96T

7.8pg/mL-500pg/mL

Less Than 1 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

For quantitative detection of human TNFsR in serum,

body fluids, tissue lysates or cell culture supernates.

1. Lyophilized recombinant human TNFsR standard: 10ng/tube×2.

2. One 96-well plate precoated with anti- human TNFsR antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- human TNFsR antibody: 130μl, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

TNF s R(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF). Chan et al. (2000) found that, in contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B; 191191) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A; 603611), CD40 (109535), and FAS (TNFRSF6; 134637), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, Milatovich et al. (1991, 1991) mapped the TNFR1 gene to 12pter-cen. Derre et al. (1991) found by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.

Human TNFsR ELISA Kit-1X96 Well Plate Image