More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TNF beta standard: 2 vials
2. One 96-well plate precoated with anti-Human TNF beta Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Tumor Necrosis Factor-β (TNF-β), also known as Lymphotoxin α (LT-α), or tumor necrosis factor ligand superfamily member 1, is a member of the TNF Superfamily. TNF-β is expressed in activated T and B lymphocytes and plays an important role in mediating lymph node development, inflammation, and immune function, in addition to its primary cytotoxic effects on tumor cells in vitro and in vivo. Natural TNF-β exists either in the secreted homotrimer or the membrane-bound hetero-trimer formed with membrane localized Lymphotoxin-β (LT-β). The homotrimetic form of TNF-β signals by binding to and activating TNFRI/TNFRSF1A, TNFRII/TNFRSF1B, and HVEM/TNFRSF14, while the heterotrimer of TNF-β acts as a ligand for the LTβR/TNFRSF3.