More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TLR3 standard: 2 vials.
2. One 96-well plate precoated with anti- Human TLR3 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100.
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Human Toll-like receptor 3 (TLR3), also known as CD283, is a type I transmembrane glycoprotein belonging to the mammalian Toll-Like Receptor family. Human TLR3 is a 904 amino acid (aa) precursor composed of a 23 aa signal sequence, a 681 aa extracellular domain (ECD) with 23 leucine rich repeats, a 21 aa transmembrane segment, and a 179 aa cytoplasmic region containing one Toll/IL1 receptor (TIR) domain. TLR3 is expressed in many different cell types and tissues including dendritic cells, macrophages, microglia, and astrocytes, as well as placenta and pancreas. TLR3 is upregulated by IFNβ and LPS. TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection. TLR3 is found in phagosomes, where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells. Ligand binding induces TLR3 dimerization and results in NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion, dendritic cell maturation and the inflammatory response.