More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TIE2 standard: 2 vials
2. One 96-well plate precoated with anti- Human TIE2 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Tie-2 (also known as Tek) is a receptor tyrosine kinase that specifically binds to angiopoietin (Ang) -1, -2, -3 and -4 which are growth factors that promote angiogenesis and the formation of blood vessels. Tie-2 is composed of two immunoglobulin (Ig)-like loops separated by three EGF-like repeats, and three fibronectin type III repeats in the extracellular domain, as well as two tyrosine kinase motifs in the intracellular fragment. Tie-2 is approximately 46% identical to Tie-1, the other member of the Tie receptor family.
Tie-2 is primarily expressed on endothelial cells (ECs) and is essential to vascular development. It is reported that Tie-2-deficient mice die at embryonic day 10.5 and exhibit significant vascular defects including abnormal dilation of the head vasculature, lack of vessel sprouting into the neuroectoderm, decreased complexity of myocardial vessels, and lower number of endothelial cells. Interaction of Tie-2 with Ang-1 can stimulate EC migration, sprout formation, and survival through activation of PI-3-Kinase. Ang-2/Tie-2 signaling leads to EC death and regression, but in the presence of VEGF can promote new vessel formation. Tie-2 is also expressed by some hematopoietic stem cells (HSCs) and neurons. In mice, Ang-1/Tie-2 can mediate the mobilization of HSCs to the peripheral circulation, and when combined with VEGF, this interaction can induce hematopoiesis and angiogenesis. Reportedly, Ang1/Tie2 signaling can protect neurons from apoptosis.