More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Survivin standard: 2 vials
2. One 96-well plate precoated with anti-Human Survivin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Survivin, also known as baculoviral IAP repeat-containing protein 5 (BIRC5) and apoptosis inhibitor 4 (API4), is a member of the inhibitor of apoptosis protein (IAP) family characterized by the baculovirus IAP repeat (BIR) domain. Structurally, Survivin is composed of a single BIR domain and an extended carboxy-terminal α-helical coil that forms a bowtie-shaped homodimer. Survivin is a component of the chromosomal passenger complex (CPC), which has been implicated in the regulation of several mitosisrelated events including chromosome alignment, histone modification, and cytokinesis. Survivin expression is cell cycle-dependent and reaches the highest level in the G2/M phase.
Survivin has exhibited its anti-apoptotic activity by inhibiting apoptotic signaling transduction, in addition to its role as a mitotic regulator. Survivin binds and inhibits caspases, the proapoptotic mitochondrial protein SMAC/Diablo, and stabilizes XIAP by preventing ubiquitination and subsequent XIAP proteasomal degradation. Survivin is significantly over-expressed in many cancers and the expression in these tumors can correlate with an increased rate of tumor recurrence and resistance to therapy. The presence of Survivin in urine has been considered as a marker for bladder cancer.