Human RANK ELISA Kit

Kit Components

 1. Recombinant Human RANK standard: 2 vials

 2. One 96-well plate precoated with anti-Human RANK Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

RANK (receptor activator of NFκB), also known as TRANCE receptor, osteoclast differentiation factor receptor (ODFR) and TNFRSF11A, is a member of the tumor necrosis factor receptor family. The full length human RANK is a 616 amino acid (aa) type I transmembrane protein containing a predicted 184 aa extracellular domain with two potential N-linked glycosylation sites and a 383 aa cytoplasmic domain. The extracellular domain of human RANK shares 81% amino acid identity with its mouse counterpart. RANK is highly expressed in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

RANK is a receptor for TRANCE (TNF related activation induced cytokines, also known as RANK ligand, RANKL), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF). RANK plays an important role in osteoclast differentiation and activation, bone remodeling and repair, immune cell function, lymph node development, thermal regulation, as well as mammary gland development. RANKL/RANK triggers TRAF-6 -mediated activation of the c-jun N-terminal kinase (JNK) and nuclear factor kappa-b (NF-kB) pathways which promote differentiation and activation of osteoclasts. Osteoprotegerin (OPG) is a decoy receptor for RANK, and regulates the stimulation of the RANK signaling pathway by competing for RANKL.