Human p53 ELISA Kit

Kit Components

 1. Recombinant Human p53 standard: 2 vials.

 2. One 96-well plate precoated with anti- Human p53 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100.

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

p53, also known as cellular tumor antigen p53, antigen NY-CO-13, phosphoprotein p53, is encoded by the TP53 gene in humans. It is a 393 amino acid (aa) protein with an apparent molecular weight of 53 kDa. p53 is characterized by an N-terminal transactivation domain, central DNA binding and oligomerization domains, and a C-terminal regulatory domain. It exhibits approximately 72% and 76% aa identity with its mouse and rat counterparts, respectively.

p53 is best known for its key role as a tumor suppressor protein. Essentially, p53 responds to signals such as DNA damage or cell stress through its actions as a transcription factor. Activated p53 binds DNA and activates expression of hundreds of genes involved in DNA repair, cell cycle regulation, or apoptosis, such as microRNA miR-34a, WAF1/CIP1 encoding for p21, etc. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a series of posttranslational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. MDM2 (Murine Double Minute 2) is a well-studied p53 regulator. It suppresses p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.