More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | <20.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human MMP-1 standard: 2 vials
2. One 96-well plate precoated with anti- Human MMP-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Matrix metalloproteinase-1 (MMP-1), also known as interstitial collagenase and fibroblast collagenase, vertebrate collagenase, or collagenase I, is a member of the Matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. MMP-1 is a 450 amino acid (aa) protein composed of an N-terminal pro domain, a catalytic domain with a zinc-binding site, a linking peptide and a C-terminal hemopexin-like domain. MMP-1 is produced by fibroblasts, chondrocytes, macrophages, keratinocytes, endothelial cells and osteoblasts, and its production is variable in response to different stimuli. MMP-1 is critical to the degradation of fibrillar collagens in extracellular matrix remodeling, it has been implicated in a variety of diseases with pathological conditions involved in collagen degradation, such as rheumatoid arthritis, osteoarthritis, periodontal disease, tumor invasion, angiogenesis, corneal ulceration, tissue remodeling, inflammatory bowel disease, atherosclerosis, aneurysm, and restenosis.