Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
| Sensitivity | < 1pg/ml |
| Size | 96T |
| Storage | Store at 4 for frequent use, at -20 for infrequent use. |
| Expiration | Four months at 4 and eight months at -20. |
| Application | serum, plasma, body fluids, tissue lysates or cell culture supernates. |
| Range | 15.6pg/ml-1000pg/ml |
More info
Product name
Human MIP-3α ELISA Kit
Catalog No.
FM-E100093
Size
96T
Range
15.6pg/mL-1000pg/mL
Sensitivity
Less Than 1pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant human MIP-3α standard: 10ng/tube×2.
2. One 96-well plate precoated with anti- human MIP-3α antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human MIP-3α antibody : 130μl, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Macrophage Inflammatory Protein 3α (MIP3α), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3α is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3α/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3α and Serine MIP-3α, the two forms of MIP3α, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3α.1 The standard product used in this kit is recombinant human MIP-3α, consisting of 70 amino acids with the molecular mass of 8KDa.
Human MIP-3α ELISA Kit-1X96 Well Plate Image