More info
Assay Range | 15.6--1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma or cell culture supernatant |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-8 standard: 2 vials
2. One 96-well plate coated with Human IL-8 Ab
3. Sample diluent buffer: 12 ml— 2
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
CXCL8, also known as Interleukin-8 (IL-8), GCP-1, and NAP-1, is a secreted protein belonging to the CXC chemokine family. IL-8 circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4. The monomer is the most bioactive form of CXCL8, while the heterodimer can potentiate PF4 activity. IL-8 oligomerization is modulated by its interactions with matrix and cell surface glycosaminoglycans (GAGs). It displays chemotactic activity for neutrophils and plays an important role in mediating innate immune system response. In addition, it also plays a role in promoting angiogenesis. CXCL8-mediated signaling pathways are transduced by two G-protein-coupled receptors, termed CXCR1/IL-8 RA and CXCR2/IL-8 RB. CXCR2 ligation induces leukocyte adhesion to activated vascular endothelium and migration to sites of inflammation, while CXCR1 ligation primes neutrophil antimicrobial activity. Clinically, CXCL8 may be related to the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.