Human IL-1beta ELISA Kit

Human IL-1beta ELISA Kit

FM-E100053

96T

3.12 pg/mL-200 pg/mL

Less Than  1 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

serum, plasma, body fluids or cell culture supernates.

 

 

1. Lyophilized recombinant human IL-1βstandard: 2ng/tube-2.

2. One 96-well plate precoated with anti- human IL-1β antibody.

3. Sample diluent buffer: 12 mL X 2

4. Biotinylated anti- human IL-1β antibody : 60µL, dilution 1:200.

5. Antibody diluent buffer: 12ml.

6. Streptavidin-HRP: 60µL, dilution 1:200.

7. Streptavidin-HRP diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

Interleukin-1β ( IL-1β) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1β, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1β and, to a lesser extent, IL-1α are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1β and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1β, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

 

Human IL-1β ELISA Kit-1X96 Well Plate Image