More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human BAFF standard: 2 vials
2. One 96-well plate precoated with anti-Human BAFF Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, tumor necrosis factor ligand superfamily member 13B (TNFSF13B) or CD257, is a type II transmembrane protein belonging to the TNF superfamily. A proteolytically processed form of BAFF has been detected in human serum. A conserved alternatively spliced isoform termed δBAFF, which can form hetero-multimers with BAFF and may negatively act to regulate BAFF secretion, has also been reported. BAFF is produced by many different cell types and tissues including monocytes, macrophages, neutrophils, dendritic cells, T lymphocytes, spleen, lymph node, and bone marrow.
BAFF is a ligand for at least three TNF receptor superfamily (TNFRSF) members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B), and BAFF receptor (BAFF R/BR3/TNFRSF13C). All three receptors are primarily expressed by B cells. BAFF R is a specific receptor for BAFF, while TACI and BCMA can bind to both BAFF and APRIL. TACI and BAFF R are cell surface receptors, however, BCMA has been found at the plasma membrane as well as in the perinuclear Golgi-like structures. BAFF may play an important role in B cell survival and maturation. It is reported that BAFF knockout mice exhibit a loss of follicular and marginal zone B cells in lymph node and spleen, while bone marrow cells and B1 cells of the peritoneum are generally unaffected. Over-expression of BAFF in mice causes an increase of B cell numbers in spleen and lymph node. These mice also exhibit characteristics of autoimmune disease including elevated levels of autoantibodies, immunoglobulin deposits in the kidneys, and glomerulonephritis accompanied by kidney dysfunction. The mechanisms by which BAFF effects on B cell survival may be attributed to regulation of anti- or pro-apoptotic members of the Bcl-2 family.