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Human Mycoplasma Pneumoniae IgG ELISA Kit

NR-R10035

$708.00

More info

Principle
Indirect ELSIA, Antigen coated plate
 
Detection Range
Qualitative Positive; Negative and Cut off
 
Size
96T
 
Specificity                      92%

Sensitivity                      95%
 
Assay time
~ 75 min
 
Shelf Life
12~14 Months from the Manufacturing Date
 
Storage
Store at 2 - 8ºC. 
 
Sample volume
10 µL Serum or Plasma
 
Detection Method
Chromogenic
  
Kit Components
 
 
 
1. 20 x Wash buffer: 1vial 
 
 
2. One 96-well plate precoated with recombinant antigen
 
 
3. Assa diluent buffer: 12 mL x 1
 
 
4. HRP conjugated: 1 vial,
 
 
5. Positive Control: 1 vial
 
 
6. Negative Control:1vial
 
 
7. Calibrator: 1 vial
 
 
8. TMB substrate: 12 ml.
 
 
9. TMB stop solution: 12 ml.

 

Background

 

Mycoplasma pneumoniae is a very small bacterium in the class Mollicutes. It is a human pathogen that causes the disease mycoplasma pneumonia, a form of atypical bacterial pneumonia related to cold agglutinin disease. M. pneumoniae is characterized by the absence of a peptidoglycan cell wall and resulting resistance to many antibacterial agents. The persistence of M. pneumoniae infections even after treatment is associated with its ability to mimic host cell surface composition. The most common sign of infection is a dry cough. Untreated or severe cases can have symptoms affecting the heart and nervous system. In rare cases, MP can be fatal. EIA serological assays are the most common method of M. pneumoniae detection used in patient diagnosis due to the low cost and relatively short testing time.

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