Human Heparin Binding Epidermal Growth Factor Like Growth Factor ( Hbegf ) ELISA Kit

Kit Components

 1. Recombinant  Human HBEGF: 2 vials

 2. One 96-well plate coated with anti-Human HBEGF Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Human HBEGF (Heparin Binding EGF-like growth factor), also known as Diphtheria toxin receptor (DT-R), is a member of the EGF family. HBEGF is synthesized as a 208 amino acid (aa) type I transmembrane preproprecursor composed of a 19 aa signal sequence, a 43 aa prosegment, an 86 aa mature region, an 11 aa juxtamembrane cleavage peptide, a 24 aa transmembrane segment, and a 25 aa cytoplasmic tail. Mature HBEGF is a soluble peptide derived from proteolytic processing of the membrane-bound form which contains an EGF-like domain and a heparin-binding motif. A series of cleaved isoforms of HBEGF and the corresponding proteases such as TACE, MMP-3, MMP- 7, ADAM-12 and ADAM-17 have been identified. HBEGF is expressed in various cell types including bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes and IL-10 secreting regulatory macrophages. HBEGF functions as a ligand for EGFR, ErbB-4, and ErbB-2. It is reported that HBEGF is required for normal cardiac valve formation and normal heart function. It is a potent mitogen for smooth muscle cells and plays a role in promoting smooth muscle cell proliferation.