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Human C-Telopeptide Of Type I Callagen CTX-1 ELISA Kit
BG-HUM10704
Human C-Telopeptide Of Type I Callagen ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general
Data sheet
| Assay time | 4.5 hours |
| Assay Range | 31.3 -- 2000 pg/mL |
| Storage | 2-8 °C |
| Assay principle | The Human CTX-1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human CTX-1 specific antibody has been pre-coated onto 96-well plate. Human CTX-1 present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human CTX-1 detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human CTX-1 captured on plate. |
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Human C-Telopeptide Of Type I Callagen ELISA Kit
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Different lot may vary, please follow the instruction coming with the kit.